The reagents consist of resorcinol and concentrated hydrochloric acid: The acid hydrolysis of polysaccharide and oligosaccharide ketoses yields simpler sugars followed by furfural.
What is the chemical composition of Seliwanoff’s reagent?
Seliwanoff Reagent
| Component Name | CAS Number | Formula |
|---|---|---|
| Resorcinol Hydrochloric acid Water | 108-46-3 7647-01-0 7732-18-5 | C6H4-1,3-(OH)2 HCl H2O |
May 16, 2014
How do you make Seliwanoff’s reagent?
Requirements. Seliwanoff's reagent: add 0.05% resorcinol (m-hydroxybenzene) in 3 N HCl. Dissolve 50 mg resorcinol in 33 ml concentrated HCl and make it 100 ml with water.
What is Seliwanoff’s reagent used for?
Seliwanoff's reagent is used to detect Fructose in seminal fluid.
How will you perform the Seliwanoff’s test given carbohydrate sample?
How to perform the test: One half ml of a sample solution is placed in a test tube. Two ml of Seliwanoff's reagent (a solution of resorcinol and HCl) is added. The solution is then heated in a boiling water bath for two minutes.
What is the function of resorcinol in Seliwanoff’s test?
Seliwanoff's test is used to distinguish aldoses from ketoses. On treatment with a concentrated Acid, ketoses are dehydrated more rapidly to give furfural derivatives and on condensation with resorcinol give cherry red complex. The test will be answered by fructose, sucrose and other keto containing carbohydrates.
What is the chemical nature of resorcinol?
Resorcinol is a benzenediol that is benzene dihydroxylated at positions 1 and 3. It has a role as an erythropoietin inhibitor and a sensitiser. It is a benzenediol and a member of resorcinols. Resorcinol is a 1,3-isomer (or meta-isomer) of benzenediol with the formula C6H4(OH)2.
How is Bial’s reagent prepared?
To prepare Bial's Reagent, add 4 drops of a 10% iron(III) chloride solution to 100 ml of 6 M hydrochloric acid. Add 0.03 g of orcinol. Stir to dissolve.